This specialized course, designed for professionals operating in the realms of electrolysis, laser, and IPL treatments, also proves beneficial for all healthcare professionals, providing the mandatory 1 hour of Board-approved Disease HIV/AIDS training as specified by the Florida Electrolysis Council. Focused on HIV/AIDS awareness and prevention, it addresses the critical need for understanding HIV/AIDS intricacies, including transmission routes, preventive measures, and the latest research findings. This knowledge is essential for ensuring safety for both clients and practitioners, particularly as these professionals engage directly with clients’ skin. The course offers an in-depth exploration of HIV/AIDS from a medical standpoint, highlighting its relevance in aesthetic practices. Through theoretical knowledge and practical guidance, participants will learn about advanced management techniques, the evolution of antiretroviral therapies (ART), and the importance of pre-exposure prophylaxis (PrEP) and post-exposure prophylaxis (PEP). Interactive modules, including case scenarios and safety protocol workshops, are designed to boost participants’ confidence in managing their practice safely and inclusively for individuals living with HIV/AIDS. This course emphasizes the significance of continuous professional development in addressing the challenges posed by blood-borne diseases within the aesthetic treatment landscape, meeting both educational and regulatory needs for a diverse range of healthcare professionals.